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KMID : 0364820100460040346
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Korean Journal of Microbiology
2010 Volume.46 No. 4 p.346 ~ p.351
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Characterization of the Biosurfactant-Producing Bacterium, Pseudoalteromonas sp. HK-3 Isolated from the Crude-Oil Contaminated Areas
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Cho Su-Hee
Oh Kye-Heon
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Abstract
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The purpose of this work was to investigate the characteristics of a biosurfactant-producing bacterium isolated from crude-oil contaminated soils. During the incubation of strain HK-3 with 1% crude-oil, bacterial growth pattern, the amount of biosurfactant production, and pH changes were monitored. In order to examine the effect of supplemented carbons on the production of biosurfactant, cultivation of HK-3 cells in BH media with different carbons (e.g. glucose, dextrose, mannitol, citrate, or acetate) revealed that the production of biosurfactant reached the maximal level at the 72 h incubation with
mannitol, which the area of clear zone was measured to approximately 7.64 cm2. Identification test using the BIOLOG system, morphology study based on scanning electron microscopy and the 16S rRNA sequence-based phylogenetic analysis assigned strain HK-3 to a Pseudoalteromonas species, designated as Pseudoalteromonas sp. HK-3 which was registered in GenBank as [FJ477041].
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KEYWORD
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biosurfactant, biosurfactant-producing bacterium, crude-oil, HK-3, Pseudoalteromonas sp
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